buffer solution
Học thuậtThân thiện
Definition
- Noun:
- A solution containing a buffer: A liquid mixture that resists significant changes in its acidity or alkalinity (pH) when small amounts of an acid or a base are added to it. It is typically composed of a weak acid and its conjugate base, or a weak base and its conjugate acid.
Usage
- Noun:
- A buffer solution is essential in many chemical and biological experiments to maintain a stable pH.
- The laboratory prepared a phosphate buffer solution for the enzyme assay.
- When you add a drop of acid to this buffer solution, you will notice very little change in the pH reading.
Advanced Usage
- "to prepare a buffer solution": To mix specific chemicals in precise proportions to create a solution with a known and stable pH.
- The protocol requires you to prepare a Tris-HCl buffer solution at pH 7.4.
- "buffer solution capacity": Refers to the amount of acid or base a buffer solution can neutralize before its pH changes significantly.
- The experiment failed because the buffer solution capacity was exceeded.
Variants and Related Words
- Buffer (n/adj): The general term for a substance or system that resists change in pH. The buffer solution is the practical application of this principle.
- The carbonate system acts as a buffer in ocean chemistry.
- Buffering (v/n): The action or process of making a solution resistant to pH change.
- The buffering ability of the solution was tested.
- Buffered (adj): Describes a solution that has been made into a buffer solution.
- Use buffered saline to wash the cells.
Synonyms
- pH-stabilized solution: A solution whose pH is maintained constant.
- Buffer system: Often used interchangeably to refer to the chemical pair that provides the buffering action within the solution.
Related Phrases
- Buffer against pH change: To protect or prevent a solution from a change in acidity.
- This compound helps buffer the cell cytoplasm against pH change.
- Run in buffer solution: To perform a process, like electrophoresis or chromatography, using a buffer solution as the medium.
- The DNA samples were run in a TAE buffer solution.
Related Concepts
- pKa: A numerical value that indicates the strength of an acid in a buffer pair; it is crucial for designing an effective buffer solution.
- Choose an acid with a pKa close to the desired pH for an effective buffer solution.
- Conjugate acid-base pair: The two components (a weak acid and its conjugate base, or vice versa) that constitute a buffer system within a buffer solution.
Noun
- a solution containing a buffer